Composite

Part:BBa_K2717009:Design

Designed by: Chenyu Liu   Group: iGEM18_BNU-China   (2018-10-09)


RBS2000-scar-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 677


Design Notes

We adjusted the codon of the last lysine of GFP from AAA to AAG.

Source

GFP and mCherry is stored in our own lab.

References